1. Can i start yaDIS when booting my Dune player? If yes how do i do that?
2. I have a System storage (Plugged in a usb on the back of my Dune Lite 53D)instaled, how do i use it, or is it used automaticcaly to cahce yaDIS videowall icons?
You don't use it, it is used by the Dune firmware for temp storage of firmware updates, storing shortcuts and the likes of BD-Live etc. You can't use it for media storage etc, you need other storage for that.